The first study on immune response to Double Helix Water was done about two decades ago, in 1998 (full study here).

This study looked at many aspects, but there are 3 questions which are frequently asked today by practitioners, which can be answered by the research done in this study.

  • Does Double Helix Water produce an immune response?

  • Is a higher dosage more effective?

  • Is there an ideal time period between doses?

Per the information in this study, the short answer to the all three questions is yes.

The initial research on the health benefits and immune support of Double Helix Water was done by Benjamin E. Bonavida, a Ph.D. Professor at UCLA who is notable for his work in Microbiology, Immunology & Molecular Genetics. Dr. Bonavida has authored over a dozen scientific books and scientific papers published in peer-reviewed scientific journals and is considered a prominent and meticulous researcher.


The results of this very first study were so startling, some found it hard to credit at the time - even though the study was done by an independent laboratory and headed by a respected, frequently published researcher.


The samples of Double Helix Water produced such a high immune response during testing it was asserted by some, that the samples had been contaminated by a drug or other factor. This was tested for and found not to be the case. All results seen in testing were purely due to Double Helix Water.


While more studies have been done since which supported the results of the initial study, this first study sparked a surprising amount of controversy, with a few orthodox and conservative researchers refusing to believe that “water” could produce these results.


In a way they were right, normal water does not. No one anywhere contests that assertion. Any observed changes in the study are due to the presence of stable water clusters (now called Double Helix Water), not water.


Now twenty years later, anyone trying to refute the existence of stable water clusters, is simply arguing with hard cold photographic and scientific evidence. That they exist is no longer contested. They can be seen with an AMF or EMF microscope and thousands of pictures have been taken.

Photo Credit: D&Y Laboratories, Inc. Atomic Force Microscope Photograph of Stable Water Clusters combining into double helix structures in ultrapure water.


Dr. Bonavida’s study examined if Double Helix Water had a significant effect on the immune system, by measuring cytokine production in whole blood. (Whole blood refers to blood drawn directly from the body without any components such as plasma removed).


The researchers used not one, but two placebos in their control group in order to eradicate any doubt concerning the validity of the results. The first placebo was clean water obtained from the laboratory where the research took place and the second placebo was distilled water.


When talking about the Immune System there is a specific type of cell that is important to understand – the T Cell.


The Immune System is a very complicated subject, as is the T Cell, but for a simple understanding one could look at this analogy.  The T Cell is like a general and scout all in one. He identifies foreign attackers, or mutated natural attackers and says, “those are the bad guys.” More than this, he actually sends a message to let everyone know, “That is a bad guy.” He then sends out a separate series of orders to create soldiers whose job is to go contain or attack the bad guys that he has “painted red.”


If everything is working properly those soldiers go on out and kill the bad guys, while leaving the good guys (the other cells that are functioning properly) alone.

Obviously we are talking about a cell here – the cell does not actually talk. But it does give orders. These orders, both the ones marking the bad guys and the ones telling what kind of soldiers to produce, are chemical orders. The T Cell creates various biological chemicals to send out his orders. There are a whole range of these biological chemicals that we know about (and can test for) and the general name for them are cytokines.


So – to recap: the T Cell identifies agents in the body that do not belong, and produces cytokines to destroy or remove those agents.


This is what a properly functioning immune system does.


THE ORIGINAL STUDY PRODUCED STARTLING RESULTS. As can be seen in the graphs here, Double Helix Water induced a significant increase in the production of Cytokines.

​TNF-A is a cytokine involved in inflammation and is important for resistance to infection and cancer.

​ IL-12 is a very important cytokine which enhances the immune response to viral and bacterial infections and induces an immune response in the body.

IL-6 is a cytokine which regulates the immune and inflammatory response, having both pro and anti-inflammatory properties.



This last graph shows what is perhaps the most interesting and important result of this study – the effect pf Double Helix Water on Interefon Y production.

IFN-Y is a cytokine critical for the immune systems defense against viral and bacterial infections.

With many types of cytokines identified by researchers, there are numerous functions a cytokine performs in the human body. For instance, some cytokines increase inflammation to help fight off “attackers,” while others lower inflammation.


The main job of an interferon in the human body is three-fold: to regulate natural immunity, to activate antiviral properties inherent in the body and to regulate cell growth (antiproliferative effects).


Interferon Y is a particularly interesting cytokine as research has shown it to be a “regulatory” cytokine. In recent studies it has been referred to as a “master checkpoint regulator.”  Meaning it seems to have the ability to regulate the production and distribution of what types of cytokines the immune system produces overall.  It lets the T Cells know what kind of other cytokines to produce.  This is very important as some cytokines increase inflammation to “kill” attackers, while others actually decrease inflammation when it has gotten “out of hand.”  So, Interferon Y is not just an “attacker” - it appears to be able to influence how the Immune System functions on a very basic level.


Yes it is. The following graphs show various concentrations being tested. The higher the concentration of Double Helix Water, the larger the immune response is.

Higher concentrations of Double Helix Water produce increased response in this study.

Different cytokines have different response rates relative to the concentration of stable water clusters. What is clear is that there is increased response to higher concentrations or quantities of stable water clusters on the cytokines tested in this study.



One of the key questions that remains to a degree unresolved is the exact dosage of Double Helix Water.

While the normal dosage recommended on the bottle serves fine for most minor issues, in some instances, medical practitioners have used much higher quantities. It may also be possible to accelerate desired results by increasing the dosage, and while we have received anecdotal reports on this from highly qualified medical practitioners, no rigorous study has been done to allow for such a claim to be made at this time.

Figures 4 and 5 provide indication on what can be expected at higher dosages. One would want to be aware of any possible herxheimer or detox reaction. Later studies show that in addition to immune response, Double Helix Water assists in moving both nutrition and intracellular waste across the cell membrane.



There is a lot more information that needs to be gathered on this subject. What we can however say is that during the original study, time tables were drawn up looking at various immune responses. Ideal times vary depending on what component of the immune system one is looking at. Our current best estimate is every 8 to 12 hours.

On Il-10 related response times we see an interval of 24 hours in order to produce a significant response.


IL-10 is also known as the “Master Regulator” of the Immune System. It is a key anti-inflammatory cytokine which regulates the inflammation brought about by T Cells when they are fighting off invading viruses and bacteria.

Figure 7: However other cytokine secretion responds much faster, and peaks at the 8 hour mark.


  • In the very first study, done by an outside laboratory, overseen by one of the world’s leading experts on immunology, Double Helix Water produced a significant response on a section of the Immune System that deals with regulating or modulating the proper function of the immune system.

  • Higher doses produced faster results, and may be required in severe cases to produce adequate response.

  • Optimum timing for dosages varies, though the earliest drop off observed in tests so far are after 8 hours intervals.



Bonavida, B. H., & Gan, X. H. (1998). Induction And Regulation Of Human Peripheral BLOODTH1-TH2 Derived Cytokines By Iewater Preparations and Synergy With Mitogens (Proceedings of the First International Symposium, Vol. Physical, Chemical and Biological Properties of Stable Water (IE™) Cluster). Los Angeles, CA: World Scientific Publishing Company. doi:

Bonavida, B., & Baritaki , S. (2012). Stable Water Clusters−Mediated Molecular Alterations in Human Melanoma Cell Lines. Forum on Immunopathological Diseases and Therapeutics, 3(3-4), 253–259. doi: 10.1615/ForumImmunDisTher.2013007833

Gee, K., Guzzo, C., Che Mat, N. F., Ma, W., & Kumar, A. (2009). The IL-12 family of cytokines in infection, inflammation and autoimmune disorders. Inflamm Allergy Drug Targets, 8(1), 40–52. doi: 10.2174/187152809787582507

Tisoncik, J. R., Simmons, M. J., Farrar, J. R., Martin, T. G., & Katze, M. undefined. (2012). Into the eye of the cytokine storm. Microbiology and Molecular Biology Reviews: MMBR, 76(1). doi:

Tisoncik, J. R., Korth, M. J., Simmons, C. P., Farrar, J., Martin, T. R., & Katze, M. G. (2017). Interferon-γ is a master checkpoint regulator of cytokine-induced differentiation. Retrieved April 14, 2020, from



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All Rights Reserved.

These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure, or prevent any disease.
Image by Denys Nevozhai


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